Rapid and simple determination of the Escherichia coli phylogenetic group.

  title={Rapid and simple determination of the Escherichia coli phylogenetic group.},
  author={Olivier Clermont and St{\'e}phane Bonacorsi and Edouard Bingen},
  journal={Applied and environmental microbiology},
  volume={66 10},
Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on triplex PCR. The method, which uses a combination of two genes (chuA and yjaA… CONTINUE READING
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