Rapid and sensitive routine detection of all members of the genus enterovirus in different clinical specimens by real-time PCR.

@article{Nijhuis2002RapidAS,
  title={Rapid and sensitive routine detection of all members of the genus enterovirus in different clinical specimens by real-time PCR.},
  author={Monique Nijhuis and Noortje M. van Maarseveen and Rob Schuurman and Sandra A. W. M. Verkuijlen and Machiel de Vos and Karin A W Hendriksen and Anton M. van Loon},
  journal={Journal of clinical microbiology},
  year={2002},
  volume={40 10},
  pages={3666-70}
}
We developed a rapid and sensitive method for the routine detection of all members of the enterovirus genus in different clinical specimens by using real-time TaqMan quantitative PCR. Multiple primer and probe sets were selected in the highly conserved 5'-untranslated region of the enterovirus genome. Our assay detected all 60 different enterovirus species tested, whereas no reactivity was observed with the viruses from the other genera of the picornaviridae family, e.g., hepatovirus and… CONTINUE READING

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