Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR.

@article{Briglia1996RapidAS,
  title={Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR.},
  author={Maria Francisca Siqueira Briglia and Rik I. L. Eggen and Willem M de Vos and Jan Dirk van Elsas},
  journal={Applied and environmental microbiology},
  year={1996},
  volume={62 4},
  pages={1478-80}
}
A method based on 16S rRNA gene-targeted PCR and oligonucleotide probing was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts of three soils. The method allowed for PCP-1 detection in soil with a detection limit of 3 x 10(2) cells per g. 

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