Rapid and sensitive liquid chromatography-tandem mass spectrometric method for the quantitation of metformin in human plasma.

@article{Wang2004RapidAS,
  title={Rapid and sensitive liquid chromatography-tandem mass spectrometric method for the quantitation of metformin in human plasma.},
  author={Yingwu Wang and Yunbiao Tang and Jingkai Gu and John Paul Fawcett and Xu Bai},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2004},
  volume={808 2},
  pages={215-9}
}
A rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS-MS) method for the determination of metformin in human plasma using phenformin as internal standard has been developed and validated. Sample preparation of plasma involved acidification with acetic acid, deproteination with acetonitrile and washing with dichloromethane. Samples were then analyzed by HPLC on a short Nucleosil C18 column (5 microm, 50 mm x 4.6 mm i.d.) using a mobile phase consisting of acetonitrile… CONTINUE READING