Rapid and sensitive determination of acetylsalicylic acid and its metabolites using reversed-phase high-performance liquid chromatography.

@article{OKruk1984RapidAS,
  title={Rapid and sensitive determination of acetylsalicylic acid and its metabolites using reversed-phase high-performance liquid chromatography.},
  author={R J O'Kruk and Mark A. Adams and Richard B. Philp},
  journal={Journal of chromatography},
  year={1984},
  volume={310 2},
  pages={
          343-52
        }
}
A rapid and sensitive high-performance liquid chromatographic technique was developed for the simultaneous determination of gentisic acid, salicyluric acid, acetylsalicylic acid and salicylic acid in plasma and serum. The method involved a single deproteinization step and separation using a reversed-phase column eluted with a buffered methanol (35%) mobile phase. Detection was achieved with a variable-wavelength ultraviolet detector set at 235 nm and a given chromatographic analysis could be… Expand
A sensitive assay for aspirin and its metabolites using reversed-phase ion-pair high-performance liquid chromatography
Abstract An ion-pair high performance liquid chromatographic (HPLC) method was developed for quantitating aspirin (ASA) and its major metabolites (salicylic acid (SA), gentisic acid (GA) andExpand
Rapid and sensitive determination of acetylsalicylic acid and salicylic acid in plasma using liquid chromatography-tandem mass spectrometry: application to pharmacokinetic study.
TLDR
The developed method was successfully applied for the evaluation of pharmacokinetics of ASA and SA after p.o. and i.v. administration of 1 mg/kg to rats. Expand
Determination of acetylsalicylic acid and its major metabolite, salicylic acid, in human plasma using liquid chromatography-tandem mass spectrometry: application to pharmacokinetic study of Astrix in Korean healthy volunteers.
TLDR
The developed assay method was successfully applied for the evaluation of pharmacokinetics of ASA and SA after single oral administration of Astrix (entero-coated pellet, 100 mg of aspirin) to 10 Korean healthy male volunteers. Expand
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Abstract A liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of acetylsalicylic acid (ASP) and its major metabolite, salicylic acid (SAL) in humanExpand
Sensitive determination of aspirin and its metabolites in plasma by LC-UV using on-line solid-phase extraction with methylcellulose-immobilized anion-exchange restricted access media.
TLDR
The method was successfully applied to an analysis of the pharmacokinetics of ASA and SA in rats and validated with respect to intra- and inter-day precision, accuracy, and linearity up to concentrations of 20,000 ng/mL for ASA, SA, 2,3-DHBA and gentisic acid, respectively. Expand
Rapid high-performance liquid chromatographic assay for salicylic acid in plasma without solvent extraction.
TLDR
In this work, a rapid and sensitive method is presented that is useful for the quantitation of salicylic acid in biological fluids and uses high-performance liquid chromatography with spectrophotometric detection for the identification and quantification of salcylic acid without organic extraction. Expand
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Abstract The salicylates are the most commonly used analgesic, antipyretic and anti-inflammatory drugs. Measurement of serum levels of salicylate is important not only in the diagnosis and managementExpand
SIMULTANEOUS DETERMINATION OF ASPIRIN AND ITS METABOLITE FROM HUMAN PLASMA BY UPLC-UV DETECTION: APPLICATION TO PHARMACOKINETIC STUDY
A rapid and sensitive liquid chromatography method with UV detection for simultaneous measurement of aspirin (ASA) and salicylic acid (SA) was developed and validated completely in human plasma. ASA,Expand
Hollow fiber liquid-phase microextraction and determination of nonsteroidal anti-inflammatories by capillary electrophoresis and sulfonamides by HPLC in human urine.
TLDR
The capillary electrophoresis with a photodiode array detection method for the analysis of seven nonsteroidal anti-inflammatory drugs (NSAIDs) and a high-performance liquid chromatographic with photo diode array and fluorescence detection Method for the determination of four sulfonamides and their corresponding N(4)-acetyl-metabolites were successfully applied. Expand
Review of HPLC methods and HPLC methods with mass spectrometric detection for direct determination of aspirin with its metabolite(s) in various biological matrices.
TLDR
This paper provides a comprehensive overview of various bioanalytical methods (HPLC and LC-MS/MS) reported for direct quantitation of aspirin along with its metabolite(s) and general information on sample collection, sample processing, internal standard selection, conditions for chromatographic separation, succinct validation data and applicable conclusions for reported assays in a structured manner. Expand
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A rapid and sensitive high-pressure liquid chromatographic assay was developed for aspirin, salicylic acid, and salicyluric acid in plasma that can be assayed directly in the presence of a large quantity of salicyric acid. Expand
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An assay is reported for salicylic acid and two of its metabolites, salicyluric acid and gentisic acid, in mouse and rat blood, urine, and feces and rat embryos. The procedure involves aqueousExpand
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TLDR
A specific and sensitive method has been developed for the determination of acetyl-salicylic Acid and salicylic acid in plasma using dibutyl maleate as the internal standard. Expand
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TLDR
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Silylation of salicylic acid by hexamethyldisilazane, N,O-bis(trimethylsilyl)acetamide, and N,N-bis(trimethylsilyl)trifluoroacetamide was compared using GLC. The completeness of the reaction, theExpand
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