Rapid and sensitive detection of shrimp yellow head virus using loop-mediated isothermal amplification and a colorogenic nanogold hybridization probe.

@article{Jaroenram2012RapidAS,
  title={Rapid and sensitive detection of shrimp yellow head virus using loop-mediated isothermal amplification and a colorogenic nanogold hybridization probe.},
  author={Wansadaj Jaroenram and Narong Arunrut and Wansika Kiatpathomchai},
  journal={Journal of virological methods},
  year={2012},
  volume={186 1-2},
  pages={36-42}
}
Salt-induced self-aggregation of gold nanoparticles (AuNP) carrying unisense ssDNA probes can be prevented specifically by complementary DNA. Loop-mediated isothermal amplification (LAMP) can amplify DNA rapidly. Here, the two techniques were combined to detect yellow head virus (YHV). The LAMP-AuNP method required 60 min for LAMP and 5 min for hybridization of LAMP products to an AuNP-labeled ssDNA probe followed by salt induced probe-particle aggregation to visualize color development. The… CONTINUE READING