Rapid and sensitive detection of retrovirus entry by using a novel luciferase-based content-mixing assay.

@article{Kolokoltsov2004RapidAS,
  title={Rapid and sensitive detection of retrovirus entry by using a novel luciferase-based content-mixing assay.},
  author={Andrey A. Kolokoltsov and Robert A Davey},
  journal={Journal of virology},
  year={2004},
  volume={78 10},
  pages={5124-32}
}
We describe a novel assay that permits measurement of entry of murine leukemia virus and pseudotypes with greater sensitivity and more rapidly than previously possible. To achieve this, we encapsulated a sensitive reporter enzyme, luciferase, directly into fully infectious, intact viral particles. The enzyme is specifically targeted to the viral lumen, as a C-terminal fusion on the viral envelope protein. Only when the incorporated luciferase is released from the viral lumen and gains access to… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 15 extracted citations

Similar Papers

Loading similar papers…