Rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV) by loop-mediated isothermal amplification combined with a lateral-flow dipstick.

@article{Nimitphak2010RapidAS,
  title={Rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV) by loop-mediated isothermal amplification combined with a lateral-flow dipstick.},
  author={Tongchai Nimitphak and Watcharachai Meemetta and Narong Arunrut and Saengchan Senapin and Wansika Kiatpathomchai},
  journal={Molecular and cellular probes},
  year={2010},
  volume={24 1},
  pages={1-5}
}
Several methods such as traditional PCR or nested-PCR, immuno assay and histopathology have been developed for detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV) formerly called monodon baculovirus (MBV). However, these methods have various disadvantages including low sensitivity, long assay time, use of toxic substances or unsuitability for field diagnosis. Loop-mediated isothermal amplification of target nucleotide sequences under isothermal conditions, combined with amplicon… CONTINUE READING