Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR.

@article{Hu1999RapidAS,
  title={Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR.},
  author={YiRan Hu and Qian-xian Zhang and J C Meitzler},
  journal={Journal of applied microbiology},
  year={1999},
  volume={87 6},
  pages={867-76}
}
Cattle are considered the major reservoir for Escherichia coli O157:H7, one of the newly emerged foodborne human pathogens of animal origin and a leading cause of haemorrhagic colitis in humans. A sensitive test that can accurately and rapidly detect the organism in the food animal production environment is critically needed to monitor the emergence, transmission, and colonization of this pathogen in the animal reservoir. In this study, a novel multiplex polymerase chain reaction (PCR) assay… CONTINUE READING