Rapid and sensitive detection of Enterobacter sakazakii by cross-priming amplification combined with immuno-blotting analysis.

@article{Yulong2010RapidAS,
  title={Rapid and sensitive detection of Enterobacter sakazakii by cross-priming amplification combined with immuno-blotting analysis.},
  author={Zhao Yulong and Zhang Li Xia and Zhang Hongwei and Liu Wei and Zheng Wenjie and Huang Xitai},
  journal={Molecular and cellular probes},
  year={2010},
  volume={24 6},
  pages={396-400}
}
Enterobacter sakazakii is a widespread and life-threatening bacterium especially in polluted powdered infant milk formula. Several methods have been developed for detection of E. sakazakii such as physiological and biochemical methods, PCR and loop-mediated isothermal amplification. However, these procedures were disadvantages due to a long assay time, low sensitivity or the use of toxic reagents. Our method of cross-priming amplification (CPA) under isothermal conditions combined with immuno… CONTINUE READING

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