Rapid and sensitive detection of Enterobacter sakazakii by cross-priming amplification combined with immuno-blotting analysis.

@article{Yulong2010RapidAS,
  title={Rapid and sensitive detection of Enterobacter sakazakii by cross-priming amplification combined with immuno-blotting analysis.},
  author={Zhao Yu-long and Z. Xia and Z. Hong-wei and L. Wei and Zheng Wen-jie and Huang Xi-tai},
  journal={Molecular and cellular probes},
  year={2010},
  volume={24 6},
  pages={
          396-400
        }
}
  • Zhao Yu-long, Z. Xia, +3 authors Huang Xi-tai
  • Published 2010
  • Biology, Medicine
  • Molecular and cellular probes
  • Enterobacter sakazakii is a widespread and life-threatening bacterium especially in polluted powdered infant milk formula. Several methods have been developed for detection of E. sakazakii such as physiological and biochemical methods, PCR and loop-mediated isothermal amplification. However, these procedures were disadvantages due to a long assay time, low sensitivity or the use of toxic reagents. Our method of cross-priming amplification (CPA) under isothermal conditions combined with immuno… CONTINUE READING
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