Rapid and sensitive detection of BRCA1/2 mutations in a diagnostic setting: comparison of two high-resolution melting platforms.

@article{Leeneer2008RapidAS,
  title={Rapid and sensitive detection of BRCA1/2 mutations in a diagnostic setting: comparison of two high-resolution melting platforms.},
  author={Kim De Leeneer and Ilse Coene and Bruce Poppe and Anne De Paepe and Kathleen Claes},
  journal={Clinical chemistry},
  year={2008},
  volume={54 6},
  pages={982-9}
}
BACKGROUND High-resolution melting is an emerging technique for detection of nucleic acid sequence variations. Developments in instrumentation and saturating intercalating dyes have made accurate high-resolution melting analysis possible and created opportunities to use this technology in diagnostic settings. We evaluated 2 high-resolution melting instruments for screening BRCA1 and BRCA2 mutations. METHODS To cover the complete coding region and splice sites, we designed 112 PCR amplicons… CONTINUE READING