Rapid and sensitive LC-MS/MS assay for the quantitation of 20(S)-protopanaxadiol in human plasma.

@article{Zhang2009RapidAS,
  title={Rapid and sensitive LC-MS/MS assay for the quantitation of 20(S)-protopanaxadiol in human plasma.},
  author={Dan Zhang and Yingwu Wang and Jiangbin Han and Weisong Yu and Lili Deng and John Paul Fawcett and Zeyuan Liu and Jingkai Gu},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2009},
  volume={877 5-6},
  pages={581-5}
}
This paper describes a rapid and sensitive method for the quantitation of 20(S)-protopanaxadiol (PPD) in human plasma based on high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analyte and internal standard (I.S.), ginsenoside Rh(2), were extracted from plasma by liquid-liquid extraction and separated on a Zorbax extend C(18) analytical column using methanol-acetonitrile-10mM ammonium acetate (47.5:47.5:5, v/v/v) as mobile phase. Detection was by tandem mass… CONTINUE READING