Rapid and reliable genotyping for the Toll-like receptor 4 A896G polymorphism using fluorescence-labeled hybridization probes in a real-time polymerase chain reaction assay.

@article{Heesen2003RapidAR,
  title={Rapid and reliable genotyping for the Toll-like receptor 4 A896G polymorphism using fluorescence-labeled hybridization probes in a real-time polymerase chain reaction assay.},
  author={Michael Heesen and Martina Wessiepe and Dagmar Kunz and Katar{\'i}na Va{\vs}{\'i}{\vc}kov{\'a} and Brunhilde Blomeke},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={2003},
  volume={333 1},
  pages={47-9}
}
BACKGROUND The Toll-like receptor 4 (TLR4) is involved in immune response to endotoxin as well as the pathogenesis of atherosclerosis. The TLR4 gene was shown to carry a single-nucleotide polymorphism (A896G). We developed a rapid-cycle polymerase chain reaction (PCR) which allows for rapid genotyping and, therefore, may be useful in clinical risk assessment. METHODS Fluorescently labeled oligonucleotide hybridization probes were designed for the LightCycler instrument. A PCR product was… CONTINUE READING
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