Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants

@article{Kang2004RapidAR,
  title={Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants},
  author={Tae-Jin Kang and Moon-sik Yang},
  journal={BMC Biotechnology},
  year={2004},
  volume={4},
  pages={20 - 20}
}
BACKGROUND DNA extraction methods for PCR-quality DNA from calluses and plants are not time efficient, since they require that the tissues be ground in liquid nitrogen, followed by precipitation of the DNA pellet in ethanol, washing and drying the pellet, etc. The need for a rapid and simple procedure is urgent, especially when hundreds of samples need to be analyzed. Here, we describe a simple and efficient method of isolating high-quality genomic DNA for PCR amplification and enzyme digestion… CONTINUE READING
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