Rapid and reliable DNA extraction and PCR fingerprinting methods to discriminate multiple biotypes of the nematophagous fungus Pochonia chlamydosporia isolated from plant rhizospheres.

@article{ManzanillaLpez2009RapidAR,
  title={Rapid and reliable DNA extraction and PCR fingerprinting methods to discriminate multiple biotypes of the nematophagous fungus Pochonia chlamydosporia isolated from plant rhizospheres.},
  author={Rosa H. Manzanilla-L{\'o}pez and Ian M Clark and Simon D. Atkins and Penny Ruth Hirsch and Brian R. Kerry},
  journal={Letters in applied microbiology},
  year={2009},
  volume={48 1},
  pages={71-6}
}
AIMS To develop a simple, rapid, reliable protocol producing consistent polymerase chain reaction (PCR) fingerprints of Pochonia chlamydosporia var. chlamydosporia biotypes for analysing different fungal isolates during co-infection of plants and nematodes. METHODS AND RESULTS DNA extracted from different P. chlamydosporia biotypes was fingerprinted using enterobacterial repetitive intragenic consensus (ERIC)-PCR. Four extraction methods (rapid alkaline lysis; microLYSIS-PLUS; DNeasy; FTA… CONTINUE READING