Rapid and real-time detection of hepatitis A virus by reverse transcription loop-mediated isothermal amplification assay.

@article{Yoneyama2007RapidAR,
  title={Rapid and real-time detection of hepatitis A virus by reverse transcription loop-mediated isothermal amplification assay.},
  author={Tetsuo Yoneyama and Tomoko Kiyohara and Noriko Shimasaki and Gen Kobayashi and Yoshinori Ota and Tsugunori Notomi and Atsuko Totsuka and Takaji Wakita},
  journal={Journal of virological methods},
  year={2007},
  volume={145 2},
  pages={162-8}
}
A one-step, single tube, real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting sequences of the untranslated region in the genome of hepatitis A virus (HAV). The RT-LAMP assay reported in this study was very simple and rapid; the HAV-specific amplification was obtained in 50 min under isothermal conditions at 62.5 degrees C by employing a set of seven primers. The RNAs of three cell-adapted HAV strains belonging to different… CONTINUE READING