Rapid and quantitative detection of homologous and non-homologous recombination events using three oligonucleotide MLPA

Abstract

Embryonic stem (ES) cell technology allows modification of the mouse germline from large deletions and insertions to single nucleotide substitutions by homologous recombination. Identification of these rare events demands an accurate and fast detection method. Current methods for detection rely on Southern blotting and/or conventional PCR. Both the… (More)

Topics

5 Figures and Tables