Rapid and onsite BOD sensing system using luminous bacterial cells-immobilized chip.

@article{Sakaguchi2007RapidAO,
  title={Rapid and onsite BOD sensing system using luminous bacterial cells-immobilized chip.},
  author={Toshifumi Sakaguchi and Yasunori Morioka and Masahiro Yamasaki and Junpei Iwanaga and Kazuhiko Beppu and Hideaki Maeda and Yasutaka Morita and Eiichi Tamiya},
  journal={Biosensors & bioelectronics},
  year={2007},
  volume={22 7},
  pages={1345-50}
}
A BOD monitoring system based on a bio-chip which immobilized luminous bacterium in micrometer-order holes were arrayed and fabricated by micro-machine techniques, was developed. The acrylic chip (3 cmx3 cm) comprises nine micro-holes (diameter: 700 microm or 1 mm, depth: 100 microm) arranged in a three by three array. Cells of the marine luminous bacterium, Photobacterium phosphoreum IFO 13896, which was grown at 15 degrees C for 15 h, were immobilized with 3% or 15% sodium alginate gel. BOD… CONTINUE READING

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