Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites.

@article{Hamoy2008RapidAI,
  title={Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites.},
  author={Igor Guerreiro Hamoy and Eduardo Jose Marcelino Vicente dos Santos and Sidney E B Santos},
  journal={Genetics and molecular research : GMR},
  year={2008},
  volume={7 1},
  pages={29-32}
}
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population. The multiplex system described can thus be considered a… CONTINUE READING