Rapid and highly sensitive electrochemical determination of alkaline phosphatase using a composite tyrosinase biosensor.

@article{Serra2005RapidAH,
  title={Rapid and highly sensitive electrochemical determination of alkaline phosphatase using a composite tyrosinase biosensor.},
  author={Beatriz Fernandez Serra and Mar{\'i}a Dolores Morales and A Julio Reviejo and Elizabeth A H Hall and Jos{\'e} Manuel Pingarr{\'o}n},
  journal={Analytical biochemistry},
  year={2005},
  volume={336 2},
  pages={289-94}
}
The use of an amperometric graphite-Teflon composite tyrosinase biosensor for the rapid monitoring of alkaline phosphatase (ALP), with no need of an incubation step and using phenyl phosphate as the substrate, is reported. Phenol generated by the action of ALP is monitored at the tyrosinase composite electrode through the electrochemical reduction of the o-quinone produced to catechol, which produces a cycle between the tyrosinase substrate and the electroactive product, giving rise to the… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…