Rapid and flexible biochemical assays for evaluating 4'-phosphopantetheinyl transferase activity.

@article{Owen2011RapidAF,
  title={Rapid and flexible biochemical assays for evaluating 4'-phosphopantetheinyl transferase activity.},
  author={Jeremy G Owen and Janine N. Copp and David F Ackerley},
  journal={The Biochemical journal},
  year={2011},
  volume={436 3},
  pages={709-17}
}
PPTases (phosphopantetheinyl transferases) are of great interest owing to their essential roles in activating fatty acid, polyketide and non-ribosomal peptide synthetase enzymes for both primary and secondary metabolism, as well as an increasing number of biotechnological applications. However, existing techniques for PPTase characterization and development are cumbersome and technically challenging. To address this, we have developed the indigoidine-synthesizing non-ribosomal peptide… CONTINUE READING