Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor.

Abstract

We have created a novel enzyme reactor using electric field-mediated orientation and immobilization of proteolytic enzymes (trypsin/chymotrypsin) on biocompatible PVDF membranes in a continuous flow-through chamber. Using less than 5min, this reactor in various enzyme combinations can produce enhanced rapid digestion for standardized prototypic proteins… (More)
DOI: 10.1016/j.jprot.2011.02.007

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Cite this paper

@article{Zhou2011RapidAE, title={Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor.}, author={Yu Zhou and Tie Yi and Sung-soo Park and Wayne Anthony Chadwick and Rong-Fong Shen and Wells W. Wu and Bronwen Martin and Stuart Maudsley}, journal={Journal of proteomics}, year={2011}, volume={74 7}, pages={1030-5} }