Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition.

@article{Somboonthum2010RapidAE,
  title={Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition.},
  author={Pranee Somboonthum and Tetsuo Koshizuka and Shigefumi Okamoto and Masaaki Matsuura and Yasuyuki Gomi and Michiaki Takahashi and Koichi Yamanishi and Yasuko Mori},
  journal={Virology},
  year={2010},
  volume={402 1},
  pages={215-21}
}
Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZalpha-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette… CONTINUE READING

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