Rapid and efficient gene modification in rice and Brachypodium using TALENs.

  title={Rapid and efficient gene modification in rice and Brachypodium using TALENs.},
  author={Qi-wei Shan and Yanpeng Wang and Kunling Chen and Zhen Liang and Jun Li and Yi Zhang and Kang Zhang and Jinxing Liu and Daniel F. Voytas and Xuelian Zheng and Yong Zhang and Caixia Gao},
  journal={Molecular plant},
  volume={6 4},
In the past few years, the use of sequence-specific nucleases for efficient targeted mutagenesis has provided plant biologists with a powerful new approach for understanding gene function and developing new traits. These nucleases create DNA double-strand breaks at chromosomal targeted sites that are primarily repaired by the non-homologous end joining (NHEJ) or homologous recombination (HR) pathways. NHEJ is often imprecise and can introduce mutations at target sites resulting in the loss of… 

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