Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target.

  title={Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target.},
  author={Claire Poyart and G. W. Le Quesne and C Boumaila and Patrick Trieu-Cuot},
  journal={Journal of clinical microbiology},
  volume={39 12},
Simple PCR and sequencing assays that utilize a single pair of degenerate primers were used to characterize a 429-bp-long DNA fragment internal (sodA(int)) to the sodA gene encoding the manganese-dependent superoxide dismutase in 40 coagulase-negative staphylococcal (CNS) type strains. The topology of the phylogenetic tree obtained was in general agreement with that which was inferred from an analysis of their 16S rRNA or hsp60 gene sequences. Sequence analysis revealed that the staphylococcal… CONTINUE READING
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