Rapid and accurate identification of species belonging to the Candida parapsilosis complex by real-time PCR and melting curve analysis.

@article{Hays2011RapidAA,
  title={Rapid and accurate identification of species belonging to the Candida parapsilosis complex by real-time PCR and melting curve analysis.},
  author={Constantin Hays and Chantal Duhamel and Vincent Cattoir and Julie Bonhomme},
  journal={Journal of medical microbiology},
  year={2011},
  volume={60 Pt 4},
  pages={477-80}
}
Candida parapsilosis is the second most frequent Candida species isolated from blood cultures. Since 2005, C. parapsilosis has been divided into three distinct species based on genetic traits: Candida parapsilosis, Candida metapsilosis and Candida orthopsilosis. The aim of this study was to develop a rapid real-time PCR assay able to distinguish these closely related species via a melting curve analysis. This identification method was optimized by using reference strains and well-characterized… CONTINUE READING