Rapid and accurate detection of SARS-CoV-2 mutations using a Cas12a-based sensing platform

  title={Rapid and accurate detection of SARS-CoV-2 mutations using a Cas12a-based sensing platform},
  author={Changsheng He and Cailing Lin and Guosheng Mo and Binbin Xi and An′an Li and Dongchao Huang and Yan Jin Wan and Feng Chen and Yufeng Liang and Qingxia Zuo and Wanqing Xu and Dongyan Feng and Guanting Zhang and Liya Han and Changwen Ke and Hongli Du and Lizhen Huang},
  journal={Biosensors \& Bioelectronics},
  pages={113857 - 113857}
The increasing prevalence of SARS-CoV-2 variants with spike mutations has raised concerns owing to higher transmission rates, disease severity, and escape from neutralizing antibodies. Rapid and accurate detection of SARS-CoV-2 variants provides crucial information concerning the outbreaks of SARS-CoV-2 variants and possible lines of transmission. This information is vital for infection prevention and control. We used a Cas12a-based RT-PCR combined with CRISPR on-site rapid detection system… 

Figures from this paper


Precision Response to the Rise of the SARS-CoV-2 B.1.1.7 Variant of Concern by Combining Novel PCR Assays and Genome Sequencing for Rapid Variant Detection and Surveillance
Two newly developed tests are able to identify and differentiate the variants of concern from regular strains of SARS-CoV-2 that have higher levels of transmission, less susceptibility to the authors' immune response, and possibly cause more severe disease than previous strains of the virus are described.
dsmCRISPR: Dual synthetic mismatches CRISPR/Cas12a-based detection of SARS-CoV-2 D614G mutation
The dsmCRIPSR method has significant potential to serve as a sensitive and specific assay for Sars-CoV-2 D614G detection and could be further extended for the detection of other SARS-Cov-2 variants of interest.
CRISPR–Cas12-based detection of SARS-CoV-2
The CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT–PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.
Development of a genotyping platform for SARS-CoV-2 variants using high-resolution melting analysis
A simple, easy-to-use genotyping method to identify SARS-CoV-2 variants using a high-resolution melting (HRM) analysis, which indicates that this HRM-based genotypesing method can identify SAR's coronavirus variants.
Detection of the SARS‐CoV‐2 D614G mutation using engineered Cas12a guide RNA
Overall, the symRNA‐Cas12a method is developed to specifically, sensitively and rapidly detect the SARS‐CoV‐2 D614G mutation.
FnCas9-based CRISPR diagnostic for rapid and accurate detection of major SARS-CoV-2 variants on a paper strip
The high specificity of Francisella novicida Cas9 (FnCas9) is repurposed to identify mismatches in the target for developing a lateral flow assay that can be successfully adapted for the simultaneous detection of SARS-CoV-2 infection as well as for detecting point mutations in the sequence of the virus obtained from patient samples.
RT-qPCR Assays for Rapid Detection of the N501Y, 69-70del, K417N, and E484K SARS-CoV-2 Mutations: A Screening Strategy to Identify Variants With Clinical Impact
This work provides low-cost RT-qPCR assays for rapid screening and molecular surveillance of mutations with potential clinical impact and allowed the detection of E484K mutation and P.2 Brazilian variant for the first time in samples from the Mexican population.
SARS-CoV-2 variants, spike mutations and immune escape
The literature on mutations of the SARS-CoV-2 spike protein, the primary antigen, is summarized, focusing on their impacts on antigenicity and contextualizing them in the protein structure, and discussed in the context of observed mutation frequencies in global sequence datasets.
opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection
The collateral activity against single-stranded DNA reporters of activated Cas12a triggered by RT-LAMP amplicon increases detection sensitivity and makes detection results observable with naked eye, and the opvCRISPR enables detection at nearly single molecule level in 45 min.
Implementation of an in-house real-time reverse transcription-PCR assay to detect the emerging SARS-CoV-2 N501Y variants
An in-house one-step real-time reverse transcription-PCR (qPCR) assay was found reliable to detect specifically the N501Y substitution and preliminarily allowed estimating 20I/501Y.V1 variant prevalence to 4% among the current SARS-CoV-2 diagnoses since January.