Rapid and Thiol-Specific High-Throughput Assay for Simultaneous Relative Quantification of Total Thiols, Protein Thiols, and Nonprotein Thiols in Cells

@inproceedings{Yang2015RapidAT,
  title={Rapid and Thiol-Specific High-Throughput Assay for Simultaneous Relative Quantification
of Total Thiols, Protein Thiols, and Nonprotein Thiols in Cells},
  author={Yang Yang and Xiangming Guan},
  booktitle={Analytical chemistry},
  year={2015}
}
Thiol groups in biological molecules play a significant role in various physiological functions and pathological conditions. Thiols are divided into two major groups: protein thiols and nonprotein thiols. Numerous methods have been reported for thiol assays. Most of these methods have been developed for glutathione, the principal nonprotein thiol, despite the fact that cellular protein thiols are more abundant than glutathione. Further, these methods usually involve a process of biological… CONTINUE READING