Rapid and Sensitive Detection of Apple stem pitting virus in Apple Trees Through RNA Amplification and Probing with Fluorescent Molecular Beacons.

@article{Klerks2001RapidAS,
  title={Rapid and Sensitive Detection of Apple stem pitting virus in Apple Trees Through RNA Amplification and Probing with Fluorescent Molecular Beacons.},
  author={Michel M Klerks and Giovanni Leone and Jennifer L Lindner and Cor D. Schoen and J. F. J. M. van den Heuvel},
  journal={Phytopathology},
  year={2001},
  volume={91 11},
  pages={1085-91}
}
ABSTRACT Currently, detection of Apple stem pitting virus (ASPV; genus Foveavirus) in apple trees for certification purposes occurs by woody indexing. This method requires a minimum of 12 to 24 weeks in greenhouse testing to up to 2 years in field testing. In this paper, the development of a single tube AmpliDet RNA system for the rapid gel-free detection of ASPV in apple tree tissues is described. The system relies on the specific amplification of the viral RNA by nucleic acid sequence-based… CONTINUE READING