Rapid and Efficient Isolation of High-Quality Small RNAs from Recalcitrant Plant Species Rich in Polyphenols and Polysaccharides

@inproceedings{Peng2014RapidAE,
  title={Rapid and Efficient Isolation of High-Quality Small RNAs from Recalcitrant Plant Species Rich in Polyphenols and Polysaccharides},
  author={Jun Peng and Zihao Xia and Ling Chen and Minjing Shi and Jinji Pu and Jianrong Guo and Zaifeng Fan},
  booktitle={PloS one},
  year={2014}
}
Small RNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), are important regulators of plant development and gene expression. The acquisition of high-quality small RNAs is the first step in the study of its expression and function analysis, yet the extraction method of small RNAs in recalcitrant plant tissues with various secondary metabolites is not well established, especially for tropical and subtropical plant species rich in polysaccharides and polyphenols. Here, we… CONTINUE READING
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