Rapid analysis of the major classes of retinoids by step gradient reversed-phase high-performance liquid chromatography using retinal (O-ethyl) oxime derivatives.

@article{Kuijk1985RapidAO,
  title={Rapid analysis of the major classes of retinoids by step gradient reversed-phase high-performance liquid chromatography using retinal (O-ethyl) oxime derivatives.},
  author={Frederik J van Kuijk and Garry J Handelman and Edward A. Dratz},
  journal={Journal of chromatography},
  year={1985},
  volume={348 1},
  pages={
          241-51
        }
}
A rapid step-gradient reversed-phase high-performance liquid chromatography (HPLC) method is presented for analysis of the major classes of retinoids in tissues. Retinal was converted into a new derivative, retinal (O-ethyl) oxime, since the standard derivative, retinaloxime, co-elutes with retinol on reversed-phase HPLC. The most abundant naturally occurring retinyl esters, retinyl palmitate and retinyl stearate, were eluted within 12 min to complete the separation. Retinoids were extracted in… CONTINUE READING