Rapid analysis of normal and abnormal cell types in human semen and testis biopsies by flow cytometry.

@article{Evenson1983RapidAO,
  title={Rapid analysis of normal and abnormal cell types in human semen and testis biopsies by flow cytometry.},
  author={Donald P Evenson and Myron R. Melamed},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1983},
  volume={31 1A Suppl},
  pages={
          248-53
        }
}
A simple, rapid procedure is described that quantitates RNA content and DNA content/chromatin condensation for each of many possible cell types and differentiation levels of the cells present in human semen. A fresh semen sample (1-6 hr postemission) or frozen sample (allowing samples to be accumulated and sent to a laboratory) is treated with a detergent solution, stained with acridine orange (AO), and measured by flow cytometry (FCM); approximately 10 minutes are required to measure 5,000… CONTINUE READING
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