Rapid analysis of long-chain glycolipids in heterocystous cyanobacteria using high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry.

@article{Bauersachs2009RapidAO,
  title={Rapid analysis of long-chain glycolipids in heterocystous cyanobacteria using high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry.},
  author={Thorsten Bauersachs and Ellen C. Hopmans and Justine Compaor{\'e} and Lucas J. Stal and Stefan Schouten and Jaap Smede Sinninghe Damst{\'e}},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2009},
  volume={23 9},
  pages={1387-94}
}
Under nitrogen-depleted conditions, N2-fixing cyanobacteria of the order Nostocales and Stigonematales differentiate vegetative cells into heterocysts. The cell envelope of these specialized cells contains unique glycolipids, consisting of a sugar moiety glycosidically bound to long-chain diols, triols and hydroxyketones. Only few reports have been published on these glycolipids in cultured cyanobacteria and none has reported them in natural environments. Here we show that heterocyst… CONTINUE READING