Rapid amplification of genomic ends (RAGE) as a simple method to clone flanking genomic DNA.

@article{Cormack1997RapidAO,
  title={Rapid amplification of genomic ends (RAGE) as a simple method to clone flanking genomic DNA.},
  author={R. S. Cormack and Imre E Somssich},
  journal={Gene},
  year={1997},
  volume={194 2},
  pages={273-6}
}
This report describes the amplification of upstream genomic sequences using the polymerase chain reaction (PCR) based solely on downstream DNA information from a cDNA clone. In this novel and rapid technique, genomic DNA (gDNA) is first incubated with a restriction enzyme that recognizes a site within the 5' end of a gene, followed by denaturation and polyadenylation of its free 3' ends with terminal transferase. The modified gDNA is then used as template for PCR using a gene-specific primer… CONTINUE READING
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