Rapid alteration of cellular redox homeostasis upon exposure to cadmium and mercury in alfalfa seedlings.

  title={Rapid alteration of cellular redox homeostasis upon exposure to cadmium and mercury in alfalfa seedlings.},
  author={Cristina Ortega-Villasante and Luis E Hern{\'a}ndez and Rub{\'e}n Rell{\'a}n-{\'A}lvarez and Francisca Fern{\'a}ndez del Campo and Ram{\'o}n O. Carpena-Ruiz},
  journal={The New phytologist},
  volume={176 1},
Here, the kinetics of oxidative stress responses of alfalfa (Medicago sativa) seedlings to cadmium (Cd) and mercury (Hg) (0, 3, 10 and 30 microm) exposure, expanding from a few minutes to 24 h, were studied. Intracellular oxidative stress was analysed using 2',7'-dichlorofluorescin diacetate and extracellular hydrogen peroxide (H(2)O(2)) production was studied with Amplex Red. Growth inhibition, concentrations of ascorbate, glutathione (GSH), homoglutathione (hGSH), Cd and Hg, ascorbate… 

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