Rapid accumulation of inositol trisphosphate reveals that agonists hydrolyse polyphosphoinositides instead of phosphatidylinositol.

@article{Berridge1983RapidAO,
  title={Rapid accumulation of inositol trisphosphate reveals that agonists hydrolyse polyphosphoinositides instead of phosphatidylinositol.},
  author={Michael John Berridge},
  journal={The Biochemical journal},
  year={1983},
  volume={212 3},
  pages={
          849-58
        }
}
  • M. Berridge
  • Published 1983
  • Biology, Medicine
  • The Biochemical journal
The agonist-dependent hydrolysis of inositol phospholipids was investigated by studying the breakdown of prelabelled lipid or by measuring the accumulation of inositol phosphates. Stimulation of insect salivary glands with 5-hydroxytryptamine for 6 min provoked a rapid disappearance of [3H]phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] and [3H]phosphatidylinositol 4-phosphate (PtdIns4P) but had no effect on the level of [3H]phosphatidylinositol (PtdIns). The breakdown of PtdIns(4,5)P2… Expand
Turnover of inositol phosphates in brain during ischemia-induced breakdown of polyphosphoinositides
TLDR
Results indicate that decapitative ischemic treatment to rats can serve as an experimental model for assessing in vivo stimulation of the receptor-mediated signal transduction mechanism related to polyphosphoinositide breakdown and subsequent turnover of inositol phosphates in brain. Expand
Relationship of polyphosphoinositide metabolism to the hormonal activation of the inset salivary gland by 5-hydroxytryptamine
TLDR
It is concluded that the primary action of 5-HT is to stimulate the hydrolysis of PIP2 into diacylglycerol and inositol trisphosphate, which may function as a second messenger to mobilize the calcium responsible for initiating some of the ionic events responsible for fluid secretion. Expand
Studies of endogenous polyphosphoinositide hydrolysis in human platelet membranes. Evidence that polyphosphoinositides remain inaccessible to phosphodiesterase in the native membrane.
TLDR
It is concluded that platelet polyphosphoinositides are present in the plasma membrane in a state where they remain inaccessible to phospholipase C, which is still fully active even at basal calcium concentrations, i.e., 10(-7) M. Expand
Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes
TLDR
It is shown here that intracellularly injected Ins(1,4,5)P3 mimics the muscarinic depolarizing chloride current in Xenopus oocytes, the first demonstration of a direct link between phosphoinositides metabolism and a neuro-transmitter-induced physiological response. Expand
Kinetic analysis of guanosine 5'-O-(3-thiotriphosphate) effects on phosphatidylinositol turnover in NRK cell homogenates.
Addition of the guanine nucleotide analogue guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to [3H]inositol-labeled NRK cell homogenates resulted in rapid breakdown of cellularExpand
Inositol Trisphosphate and Calcium Mobilization
  • M. Berridge
  • Chemistry, Medicine
  • Journal of cardiovascular pharmacology
  • 1986
TLDR
Structural-activity studies reveal that the vicinal phosphates on the 4- and 5-positions are essential for releasing calcium whereas the phosphate on the opposite side enhances the affinity of Ins1,4,5P3 for its putative receptor. Expand
Inositol trisphosphate and calcium mobilization.
  • M. Berridge
  • Chemistry, Medicine
  • Ciba Foundation symposium
  • 1986
TLDR
Structural-activity studies reveal that the vicinal phosphates on the 4- and 5-positions are essential for releasing calcium whereas the phosphate on the opposite side enhances the affinity of Ins1,4,5P3 for its putative receptor. Expand
Agonist-stimulated phosphatidylinositol 4,5-bisphosphate metabolism in the nervous system
  • C. Downes
  • Medicine, Biology
  • Neurochemistry International
  • 1986
TLDR
Phosphatidylinositol 4,5-bisphosphate breakdown, detected as the accumulation of inositol phosphates in agonist-stimulated nervous tissue preparations, is a functional response that has been useful in assessing the relevance of receptors identified by radioligand binding assays, and provides an essential link between receptor occupation and responses such as neurotransmitter release and modulation of neuronal excitability. Expand
Inositol phospholipid hydrolysis may mediate the action of proctolin on insect visceral muscle
TLDR
The results indicate that the locust oviducts possess proctolin receptors that are linked to phosphatidylinositol metabolism and that inositol phospholipid hydrolysis may mediate the physiological action of Proctolin. Expand
Sustained elevation in inositol 1,4,5-trisphosphate results in inhibition of phosphatidylinositol transfer protein activity and chronic depletion of the agonist-sensitive phosphoinositide pool.
TLDR
It is indicated that chronic elevation in cellular Ins(1,4,5)P(3) concentrations decreases the PITP-mediated resupply of phosphoinositides in the caveolin-enriched agonist-sensitive pool. Expand
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