Rapid Virological Diagnosis of Central Nervous System Infections

Abstract

264 words): 1 Viruses are the main etiological cause of the central nervous system (CNS) infections. A 2 rapid molecular diagnosis is recommended to improve the therapeutic management of 3 patients. The aim of this study was to evaluate the performances of a DNA microarray, the 4 CLART ® Entherpex kit (Genomica ® , Coslada, Spain), allowing the rapid and simultaneous 5 detection of 9 DNA and RNA neurotropic viruses: HSV-1, HSV-2, VZV, CMV, EBV, HHV6 6, HHV-7, HHV-8 and the human enteroviruses (HEVs). This evaluation was performed with 7 28 samples from the European proficiency panels (QCMD, Glasgow, Scotland) and then with 8 78 cerebrospinal fluid (CSF) specimens. The majority of the QCMD results obtained by the 9 DNA microarray were similar to those recorded by the overall QCMD participants. The main 10 discrepant results were observed for low concentrations of HSV-2 and HEVs. From the 11 clinical samples, the kit detected 27 of the 28 herpesviruses CNS infections and all the 30 12 HEVs positive CSF samples. No false positive result was observed among the 20 virus 13 negative CSF samples. The clinical sensitivity, specificity, negative and positive predictive 14 values of the assay were 98.3, 100, 95.2, and 100%, respectively, when the results were 15 compared to those of commercially available PCR assays. Interestingly, HHV-7 was detected 16 in 11 (37%) of the 30 HEVs positive CSF samples from children suffering from aseptic 17 meningitis causing significant longer length of stay at the hospital than infection with HEVs 18 alone (2.4 vs 1.4 days; P=0.038). In conclusion, this preliminary study showed that this DNA 19 microarray could be a valuable molecular diagnostic tool for single and mixed DNA and RNA 20 virus infections of the CNS. 21 22 23

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@inproceedings{Lvque2011RapidVD, title={Rapid Virological Diagnosis of Central Nervous System Infections}, author={Nicolas L{\'e}v{\^e}que and Adrien Van Haecke and Fanny Renois and David Boutolleau and Laurent Andr{\'e}oletti}, year={2011} }