Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers

  title={Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers},
  author={Nathan A. Baird and P. Etter and T. S. Atwood and Mark C. Currey and Anthony Shiver and Z. Lewis and E. Selker and W. Cresko and E. Johnson},
  journal={PLoS ONE},
Single nucleotide polymorphism (SNP) discovery and genotyping are essential to genetic mapping. There remains a need for a simple, inexpensive platform that allows high-density SNP discovery and genotyping in large populations. Here we describe the sequencing of restriction-site associated DNA (RAD) tags, which identified more than 13,000 SNPs, and mapped three traits in two model organisms, using less than half the capacity of one Illumina sequencing run. We demonstrated that different marker… Expand
2,564 Citations
SNP discovery and genotyping for evolutionary genetics using RAD sequencing.
  • 308
SNP discovery and genetic mapping using genotyping by sequencing of whole genome genomic DNA from a pea RIL population
  • 52
  • PDF
Multiplexed shotgun genotyping for rapid and efficient genetic mapping.
  • 365
  • PDF
Methods for Genotyping-by-Sequencing.
  • 23
Linkage Mapping and Comparative Genomics Using Next-Generation RAD Sequencing of a Non-Model Organism
  • 276
  • PDF
Genome Wide Sampling Sequencing for SNP Genotyping: Methods, Challenges and Future Development
  • 42
  • Highly Influenced
  • PDF
De novo SNP discovery and genetic linkage mapping in poplar using restriction site associated DNA and whole-genome sequencing technologies
  • 25
  • Highly Influenced


Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA (RAD) markers.
  • 784
  • PDF
High-resolution, high-throughput SNP mapping in Drosophila melanogaster
  • 54
  • Highly Influential
Construction of a genetic linkage map in man using restriction fragment length polymorphisms.
  • 6,954
  • PDF
Rapid mapping of zebrafish mutations with SNPs and oligonucleotide microarrays.
  • 123
  • PDF
Diversity Arrays Technology (DArT) for whole-genome profiling of barley.
  • P. Wenzl, J. Carling, +4 authors A. Kilian
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2004
  • 533
  • PDF