Rapid Purification and Characterization of Mutant Origin Recognition Complexes in Saccharomyces cerevisiae

Abstract

Purification of the origin recognition complex (ORC) from wild-type budding yeast cells more than two decades ago opened up doors to analyze the initiation of eukaryotic chromosomal DNA replication biochemically. Although revised methods to purify ORC from overproducing cells were reported later, purification of mutant proteins using these systems still… (More)
DOI: 10.3389/fmicb.2016.00521

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@inproceedings{Kawakami2016RapidPA, title={Rapid Purification and Characterization of Mutant Origin Recognition Complexes in Saccharomyces cerevisiae}, author={Hironori Kawakami and Eiji Ohashi and Toshiki Tsurimoto and Tsutomu Katayama}, booktitle={Front. Microbiol.}, year={2016} }