Rapid Publications Cloning and Functional Expression of the Human Islet GLP-1 Receptor Demonstration That Exendin-4 Is an Agonist and Exendin-( 939 ) an Antagonist of the Receptor

A complementary DNA for a glucagon-like peptide-1 receptor was isolated from a human pancreatic islet cDNA library. The isolated clone encoded a protein with 90% identity to the rat receptor. In stably transfected fibroblasts, the receptor bound [I]GLP-1 with high affinity (K^ = 0.5 nM) and was coupled to adenylate cyclase as detected by a GLP-1-dependent… CONTINUE READING