Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.

@article{Natsume2016RapidPD,
  title={Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.},
  author={Toyoaki Natsume and Tomomi Kiyomitsu and Yumiko Saga and Masato T Kanemaki},
  journal={Cell reports},
  year={2016},
  volume={15 1},
  pages={
          210-218
        }
}
Studying the role of essential proteins is dependent upon a method for rapid inactivation, in order to study the immediate phenotypic consequences. Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi, but its application to human cells has been limited by the difficulties of tagging endogenous proteins. We have developed a simple and scalable CRISPR/Cas-based method to tag endogenous proteins in human HCT116 and mouse embryonic stem (ES) cells by… CONTINUE READING
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