Rapid Production of Transgenic Wheat Plants by Direct Bombardment of Cultured Immature Embryos


We have developed an improved protocol for the rapid and efficient production of transgenic wheat. Three plasmids, each containing the selectable bar gene for resistance to the herbicide Basta and the β-glucuronidase (GUS) reporter gene, were delivered via particle bombardment, directly into immature embryos of two spring and one winter cultivar of wheat… (More)
DOI: 10.1038/nbt1293-1553


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