Rapid Microfluidic Double-Jump Mixing Device for Single-Molecule Spectroscopy.

@article{Dingfelder2017RapidMD,
  title={Rapid Microfluidic Double-Jump Mixing Device for Single-Molecule Spectroscopy.},
  author={Fabian Dingfelder and Bengt Wunderlich and Stephan Benke and Franziska Zosel and Niels Zijlstra and Daniel Nettels and Benjamin Schuler},
  journal={Journal of the American Chemical Society},
  year={2017},
  volume={139 17},
  pages={6062-6065}
}
We introduce a microfluidic double-jump mixing device for investigating rapid biomolecular kinetics with confocal single-molecule spectroscopy. This device enables nonequilibrium dynamics to be probed, e.g., transiently populated intermediates that are inaccessible with existing single-molecule approaches. We demonstrate the potential and reliability of the… CONTINUE READING