Rapid LC-MS / MS assay for the evaluation of hydroxyl radical generation and oxidative stress induction in vivo in rats

A new high-throughput liquid chromatography coupled with mass spectrometry (LC-MS/MS) method for the quantification of 2,3-dihydroxybenzoic acid (2, 3-DHBA) and 2,5dihydroxybenzoic acid (2,5-DHBA) i n rat plasma was developed and validated. The separation was performed on a Luna HILIC column under isocrati conditions using a 94:6 (v/v) mixture of acetonitri… CONTINUE READING