Rapid Immunohistochemistry for Cytokeratin in the Intraoperative Evaluation of Sentinel Lymph Nodes for Metastatic Breast Carcinoma

@article{Beach2003RapidIF,
  title={Rapid Immunohistochemistry for Cytokeratin in the Intraoperative Evaluation of Sentinel Lymph Nodes for Metastatic Breast Carcinoma},
  author={Robyn A Beach and Diane Lawson and Sandra M. Waldrop and Cynthia Cohen},
  journal={Applied Immunohistochemistry \& Molecular Morphology},
  year={2003},
  volume={11},
  pages={45-50}
}
The sensitivity and specificity of detecting metastatic breast carcinoma in sentinel lymph nodes using a rapid immunohistochemistry technique was determined and compared with methods currently used at the authors' institution. At the time of intraoperative consultation, after routine diagnostic touch preparations and frozen sections were prepared, 6-&mgr;m frozen sections of 72 sentinel lymph nodes from 32 patients with breast carcinoma were placed on plus slides, fixed in cold acetone for 2 or… 
Rapid Intraoperative Immunohistochemical Evaluation of Sentinel Lymph Nodes for Metastatic Breast Carcinoma
TLDR
Zymed RIHC is a sensitive method for detecting breast cancer metastases in SLNs and the speed, accuracy, and ease of interpretation of the test allow for recognition of micrometastases that might otherwise be undetectable by current methods of intraoperative evaluation.
Quantitative Molecular Analysis of Sentinel Lymph Node May Be Predictive of Axillary Node Status in Breast Cancer Classified by Molecular Subtypes
TLDR
It is confirmed that OSNA assay can provide standardized and reproducible results and that it represents a fast and quantitative tool for intraoperative evaluation of sentinel lymph node.
False-Negative Interpretation of Breast Sentinel Lymph Node Touch Preps: Analysis of the Causes with Suggestions to Improve Diagnostic Accuracy
TLDR
TPs with scant cellularity, unsatisfactory TP’s and failure to identify tumor cells with low nuclear grade were found to significantly contribute to false-negative interpretations and it is suggested that an additional TP or frozen section may be necessary if the cellularity of the initial TP is limited.
Frozen section is superior to imprint cytology for the intra-operative assessment of sentinel lymph node metastasis in Stage I Breast cancer patients
TLDR
It is suggested that frozen section is superior to imprint cytology for the intra-operative determination of sentinel lymph node metastasis in stage I breast cancer patients.
Intra-operative imprint cytology for assessing the sentinel node in breast cancer: results of its routine use over 8 years.
  • K. Contractor, M. Gohel, +5 authors H. Singhal
  • Medicine
    European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology
  • 2009
TLDR
Routine use of intra-operative imprint cytology for analysis of the SLN in breast cancer allows complete axillary surgery during a single anaesthetic for a majority of patients undergoing SLN biopsy.
Prospective Study of 162 Sentinel Lymph Node Biopsies in Breast Cancer: Usefulness of Ultrasonography in Patients Selection
TLDR
This study compared the results obtained with the different injection, intraoperative sentinel node evaluation, and postoperative evaluation to determine the reliability of SLN to predict the regional lymph node status to compare with previous studies.
Accuracy of Intraoperative Frozen Section of Sentinel Lymph Nodes After Neoadjuvant Chemotherapy for Breast Carcinoma
TLDR
FS is a reliable modality to assess the presence of SLN metastases in NAC treated patients and its limitations are highlighted, highlighting the inherent technical limitations of FS and histologic under-sampling of tissue which leads to most FN results.
Reliability of Whole Sentinel Lymph Node Analysis by One-Step Nucleic Acid Amplification for Intraoperative Diagnosis of Breast Cancer Metastases
TLDR
Intraoperative OSNA assay performed on the whole SLN gave objective and reproducible results that were useful for directing decisions regarding axillary dissection and for accurately defining the SLN stage.
Intraoperative imprint cytology of sentinel lymph nodes in breast cancer: initial experience and lessons learnt in establishing a new practice
Aims: The initial 18 months experience of performing intraoperative imprint cytology for patients with breast cancer undergoing sentinel lymph node biopsy is described for a single institution. The
A prospective study comparing touch imprint cytology, frozen section analysis, and rapid cytokeratin immunostain for intraoperative evaluation of axillary sentinel lymph nodes in breast cancer
TLDR
The authors prospectively compared touch imprint cytology with frozen section analysis and rapid cytokeratin immunostaining of SLNs for the intraoperative evaluation of disease and compared the results with final pathologic examination (FP).
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