Rapid Identification of Known and New RNA Viruses from Animal Tissues

  title={Rapid Identification of Known and New RNA Viruses from Animal Tissues},
  author={Joseph G. Victoria and Amit Kapoor and Kent W Dupuis and David P. Schnurr and Eric L Delwart},
  journal={PLoS Pathogens},
  pages={406 - 428}
Viral surveillance programs or diagnostic labs occasionally obtain infectious samples that fail to be typed by available cell culture, serological, or nucleic acid tests. Five such samples, originating from insect pools, skunk brain, human feces and sewer effluent, collected between 1955 and 1980, resulted in pathology when inoculated into suckling mice. In this study, sequence-independent amplification of partially purified viral nucleic acids and small scale shotgun sequencing was used on… CONTINUE READING
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