Rapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling.

@article{DimayacyacEsleta2015RapidHR,
  title={Rapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling.},
  author={Baby Rorielyn T Dimayacyac-Esleta and Chia-Feng Tsai and Reta Birhanu Kitata and Pei-Yi Lin and Wai-Kok Choong and Tai-Du Lin and Yi-Ting Wang and Shao-Hsing Weng and Pan Yang and Susan dR Arco and Ting-Yi Sung and Yu-Ju Chen},
  journal={Analytical chemistry},
  year={2015},
  volume={87 24},
  pages={12016-23}
}
Membrane proteins are crucial targets for cancer biomarker discovery and drug development. However, in addition to the inherent challenges of hydrophobicity and low abundance, complete membrane proteome coverage of clinical specimen is usually hindered by the requirement of large amount of starting materials. Toward comprehensive membrane proteomic profiling for small amounts of samples (10 μg), we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip… CONTINUE READING
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