Rapid Generation of Multiple Loci-Engineered Marker-free Poxvirus and Characterization of a Clinical-Grade Oncolytic Vaccinia Virus

@inproceedings{Guo2017RapidGO,
  title={Rapid Generation of Multiple Loci-Engineered Marker-free Poxvirus and Characterization of a Clinical-Grade Oncolytic Vaccinia Virus},
  author={Zong Sheng Guo and Zuqiang Liu and Magesh Sathaiah and Jiahu Wang and Roshni Ravindranathan and Eun Jung Kim and Shaohua Huang and Thomas W. Kenniston and John Cameron Bell and Herbert J. Zeh and Lisa H Butterfield and Andrea A. Gambotto and David L. Bartlett},
  booktitle={Molecular therapy. Methods & clinical development},
  year={2017}
}
Recombinant poxviruses, utilized as vaccine vectors and oncolytic viruses, often require manipulation at multiple genetic loci in the viral genome. It is essential for viral vectors to possess no adventitious mutations and no (antibiotic) selection marker in the final product for human patients in order to comply with the guidance from the regulatory… CONTINUE READING