Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays

@inproceedings{Wilham2010RapidEQ,
  title={Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays},
  author={Jason M. Wilham and Christina D Orr{\'u} and Richard A. Bessen and Ryuichiro Atarashi and Kazunori Sano and Brent Race and Kimberly D. Meade-White and Lara M. Taubner and Andrew G. Timmes and Byron Caughey},
  booktitle={PLoS pathogens},
  year={2010}
}
A major problem for the effective diagnosis and management of prion diseases is the lack of rapid high-throughput assays to measure low levels of prions. Such measurements have typically required prolonged bioassays in animals. Highly sensitive, but generally non-quantitative, prion detection methods have been developed based on prions' ability to seed the conversion of normally soluble protease-sensitive forms of prion protein to protease-resistant and/or amyloid fibrillar forms. Here we… CONTINUE READING

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